Thermo Fisher Scientific ⢠7335 Executive Way ⢠Frederick, MD 21704 ⢠thermofisher.com Results The Baculovirus Expression Vector System ... Scalable production of rAAV2 and rAAV6 in Sf9 cells. Do ZERO BIAS - scores, article reviews, protocol conditions and more Inoculum Inoculum should be grown to a density of 4.0 - 5.0 x 105 cells per mL in a non-humidified, non-gas-regulated environment. Thermo Fisher sf9 cells Gibco Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins The cells originated at the USDA Insect Pathology Laboratory from the parental IPLBSF 21 Sf21 cell line which was derived from the pupal ovarian tissue of the fall army worm Spodoptera frugiperda Gibco Sf9 cells frozen in Grace s Insect ⦠Culture mode Sf9 insect cells can be grown using any of the three This does not seem to affect growth. Sf9 cells were plated/well in a 6-well plate on Sf-900 II SFM. ZERO BIAS - ⦠Characterization of ExpiFectamine⢠Sf Transfection Reagent and baculovirus generation Figure 2. The Sf9 cell line (Thermo Fisher Scientific, Waltham, MA, USA) was cultured in Sf-900 II serum-free medium (SFM; Thermo Fisher Scientific). The cells were stained with X-glucuronide 72 hours post-transfection to detect β-gluc expression. Our mission is to enable our customers to make the world healthier, cleaner and safer. Clumps can be broken up during passage. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more The following morphological changes are typical of monolayer Sf9 cells infected with recombinant AcNPV. Thermo Fisher Scientific Our lab uses serum-free and supplemented SF-900 II media for the maintenance of Sf9 cells in suspension. For the best recovery and viability, thaw Sf9 cells into complete TNM-FH (TNM-FH containing 10% FBS). Supplier Page Sign In or Register to view pricing. Thermo Fisher sf9 insect cell line Sf9 Insect Cell Line, supplied by Thermo Fisher, used in various techniques. For more information, refer to www.lifetechnologies.com or contact Technical Support (page 45). Bioz Stars score: 99/100, based on 5 PubMed citations. ⢠It is critical that cell viability be at least 90% and the growth rate be in mid-logarithmic phase prior to initiating adaptation procedures. ExpiSf Expression System: A Chemically defined baculovirus-based expression system for enhanced protein and virus production in Sf9 cells In this video, we describe the ExpiSf Expression System, the first chemically defined insect system that enables high-yield production of proteins and viral particles at small and large scales. The ExpiSf9 cell line (Thermo Fisher Scientific) was cultured in ExpiSf CD medium (Thermo Fisher Scientific). Sf9 cells in Sf-900 II SFM from Thermo Fisher Scientific. High Five⢠cells may be ⦠Grace S Insect Cell Culture Medium, supplied by Thermo Fisher, used in various techniques. Sf9 cells in Sf-900 II SFM Thermo Fisher Scientific Quantity: 1.5 mL; Reviews: Write a Review. mammalian cell culture medium, medium for insect cell cultures is temperature-sensitive and should be added after sterilization of the culture vessel. The meta-bolic condition of the culture and growth medium used also can affect the timing of baculovirus infection. Figure 1. rAAV production in Sf9 cells. In this case, High Five⢠cells produced 5-fold more SEAP than Sf9 cells and 8-fold more than Sf21 (Davis, et al., 1993). Recombinant protein production was used as the basal medium. Baculovirus Production and Titering, Bioproduction, Cell Culture, Insect Cell Culture, Insect Expression, Insect Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis, Sf9 and Sf21 Cell Culture, Viral Vaccine Production. The Sf21 cell line was isolated from primary explants of Spodoptera frugiperda pupal tissue in hemolymph-supplemented medium (Vaughn et al., 1977) and Sf9 was derived from Sf21 cells (Summers and Smith, 1987). Characteristics of ExpiSf9 CD Medium cell culture Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells, 1Thermo Fisher Scientific, Inc., 7335 Executive Way, Frederick, MD 21704 2Thermo Fisher Scientific, Inc., 5781 Van Allen Way, Carlsbad, CA 92008 ExpiSf⢠Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells Figure 1. Sf9 Cells In Sf 900 Ii Sfm, supplied by Thermo Fisher, used in various techniques. 1. Compare Product. Sf9 cells are regular in size, easy to manipulate, and form good monolayers for plaque assays. Sf21 cells and 23-fold more protein than Sf9. Both cell lines are commercially available (Sigma St. Louis, MO USA; Thermo Fisher Scientific Waltham, MA, USA). ⢠We recommend using a sequential adaptation protocol. Cells and Culture Medium: Sf9 cells were adapted to HyClone SFM4Insect (Thermo Fisher Scientific, SH30913), a serum-free, protein-free and ADCF medium developed to support baculovirus. Bioz Stars score: 99/100, based on 500 PubMed citations. Typically, insect cells are unable to process N-glycans to the extent that mammalian cells do. One hour later, the cells were transfected with bacmid DNA coding for the β-glucuronidase gene, using Cellfectin ® Reagent. 1. Cell line selection . Product Amount Cat. Sf9 Frozen Cells 1 ml tube, 1 × 107 cells/ml B825-01 Sf21 Frozen Cells 1 ml tube, 1 × 107 cells/ml B821-01 High Five⢠Cells 1 ml tube, 3 × 106 cells/ml B855-02 Sf9 Cells Transfected with Cellfectin ® Reagent Figure 3. Mimic Sf9 Insect Cells are a derivative of the Sf9 insect cell line. These cells have been modified to stably express a variety of mammalian glycosyltransferases. » 9600 - Thermo Life Science » Gibco kliknutím zobrazíte plný náhled Kód 11496015 Výrobce Gibco Skladem Pardubice 0 ks MJ ks DPH 21% Cena bez DPH 16 870,00 CZK Cena vÄetnÄ DPH 20 412,70 CZK Vložit do koÅ¡íku no. Grace S Insect Cell Medium, supplied by Thermo Fisher, used in various techniques. This product(s) resides on a Fisher ⦠Sf9 cells were maintained in SF900 III medium (Thermo Fisher) at 28 °C and transfected by plasmid DNA (pSLI/V5-His-NbPTP1 and pSLI/V5-His) using X-tremeGENE HP DNA Transfection Reagent (Roche). Cell line selection We recommend Sf9 or Sf21 cells for transfection, purification, and amplification of recombinant virus. S2 cells should be subcultured to a final density of 2 × 106â4 × 106 cells/mL. Thermo Fisher Scientific ⢠7335 Executive Way⢠Frederick,MD 21704⢠thermofisher.com I.Consistency of ExpiSf⢠CD Medium in Sf9 Cell Culture Figure 4. Other Cell Lines You may also use Sf21 or High Five⢠cells as a host for pIZT/V5-His. Bioz Stars score: 99/100, based on 8 PubMed citations. (A) AAV genome titers of crude lysates from ExpiSf9 cells and Sf9 cells in Sf-900â¢II SFM. Bioz Stars score: 99/100, based on 8 PubMed citations. Cells were maintained at ⦠Sf21 cells are larger and may produce more protein than Sf9 cells. This can affect protein structure, function, antigenicity, and enzymatic activity. The World Leader in Serving Science Thermo Fisher Scientific is the world leader in serving science, with revenues of more than $20 billion and approximately 70,000 employees globally. Mimicâ¢Sf9 cells for protein expression studies. (A) AAV genome titers of crude lysates from ExpiSf9 cells cultured in ExpiSf CD medium. We recommend Sf9 or Sf21 cells for transfection, purification, and amplification of recombinant virus. Sf9 Insect Cells, supplied by Thermo Fisher, used in various techniques. Since High Five⢠cells are larger than Sf9 or Sf21 cells and the assays were performed on adherent cells, the amount of SEAP was also determined per milliliter of culture medium. infection cycle and the changes in cell morphology vary with the insect cell line and strain of baculovirus used. In this video, we describe the ExpiSf Expression System, the first chemically defined insect system that enables high-yield production of proteins and viral particles at small and large scales. As controls, rAAV production runs were performed in the absence of enhancer. (B) rAAV genome titers of crude lysates from Sf9 cells seeded at two different densities (1 x 10ⶠand 2 x 10ⶠcells/mL) in Sf-900 II medium. The culture medium was changed at 6 h post transfection. 1Thermo Fisher Scientific, Inc., 7335 Executive Way, Frederick, MD 21704 2Thermo Fisher Scientific, Inc., 5781 Van Allen Way, Carlsbad, CA 92008 ExpiSf⢠Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells Figure 1. Cells will start to clump at a density of ~5 × 106 cells/mL in serum-containing medium. Sf9 cells are regular in size, easy to manipulate, and form good monolayers for plaque assays. Sf9 Cells Synovial Fluid Spliceosomes Cell Line Synovial Membrane Cells, Cultured HeLa Cells Carpus, Animal Fibroblasts Knee Joint Tumor Cells, Cultured Cell Line, Tumor Cell Nucleus Cell Membrane Cartilage, Articular Joints T-Lymphocytes Virion Gonads CHO Cells Limb Buds Liver Leukocytes, Mononuclear Testis COS Cells Sertoli Cells 3T3 Cells Guidelines to adapt Sf9 and Sf21 cells to ExpiSf⢠CD Medium ⢠You may adapt your Sf9 and Sf21 cells for growth in ExpiSf⢠CD Medium. Description Gibco Sf9 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Line ( Thermo Fisher, used in various techniques clump at a of... Best recovery and viability, thaw Sf9 cells are larger and may produce more protein than Sf9 in! Supplied by Thermo Fisher Scientific Sf9 or Sf21 cells are unable to process to... Be ⦠Sf9 cells in Sf 900 Ii SFM from Thermo Fisher, used in various techniques AAV... 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