7. 25:33. P0, P1, and P2 signify non-amplified (P0) or amplified (P1, P2) baculovirus stock used for the respective groups. PFU were determined with a standard plaque assay (6). The Expression system format in the most complete kit available; these kits include pFastBac or pFastBac TOPO cloning vectors, specialized competent cells (DH10Bac E coli) for bacmid generation, and our ExpiFectamine Sf Transfection Reagent. The BaculoDirect™ system accelerates research by eliminating these time-consuming steps, saving you precious hands-on time. We manufacture ExpiSf CD Medium in cGMP-compliant facilities and offer custom packaging to scale with your development, so you can be confident that our product quality enhances yours. The baculovirus vector system was originally developed for the production of … Numerous technological improvements, including progress in vector design, simplification of virus isolation techniques, and … Bac-to-Bac™ Baculovirus Expression System USER GUIDE An efficient site-specific transposition system to generate baculovirus for high-level expression of recombinant proteins Catalog Numbers 10359-016, 10360-014, 10584-027, 10712-024 Publication Number MAN0000414 Revision B.0. Comparative western blotting analysis of a membrane protein (top panel), a receptor-type protein kinase (middle panel) and a tyrosine-protein kinase (bottom panel) produced in ExpiSf and control (Sf9 cells in Sf-900 II SFM) is shown. *not included in China ** not included in all regions. Bac-to-Bac® Baculovirus Expression System An efficient site-specific transposition system to generate baculovirus for high-level expression of recombinant proteins Catalog Numbers 10359-016, 10360-014, 10584-027, 10712-024 Document Part Number 10359 Publication Number MAN0000414 Revision A.0 . We provide an overview of the virus structure and its unique bi-phasic replication cycle, which has been exploited in developing the virus as an expression vector. In contrast, the Bac-to-Bac Baculovirus Expression System is faster and easier because the technology relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells, which often results in low titers. The Baculovirus Expression Vector System (BEVS) is an established method within the scientific and industrial communities for generating large quantities of recombinant protein. Here, we present data on the performace of a novel Sf9-based Baculovirus expression system based upon a yeastolate-free, animal origin-free, chemically-defined, high-density culture medium that allows for Sf9 cells to reach densities nearly twice as high as those attained in traditional yeastolate-containing media. ExpiSf Expression System: A Chemically defined baculovirus-based expression system for enhanced protein and virus production in Sf9 cells. Baculoviridae is a family of viruses. 2. The aim of this study was to produce recombinant N protein of IBV in the baculovirus system to use in ELISA diagnostic tests in order to enable the assessment of the sero-prevalence and risk of IBV infections in chickens in Turkey. Engineering the pFastBac vector with blunt TOPO Cloning technology simplifies your cloning reaction to a 5-minute bench-top reaction compared to tedious and time-consuming restriction enzyme cloning, which can take 2–3 days of work. The system includes the first chemically defined insect cell growth medium for superior run-to- run reproducibility. Cost show is based on list price in USD. 2009;463:191-222. doi: … Manufacturer Thermo Fisher Scientific | Available Worldwide. Créer un compte, A3767801,A3767802,A3767803,A3767804,A3767805,A35243,A38915,A3767806,A3767807,A3767808,10360014,10361012,31985062,4115-0125,A3767809, C404003,11593027,15160054,15750060,15519028,15529019,12532016, 10584027,10712024,10359016,A11100,A11101,A11339,A11098,A11099,A11338,F2915,17-6991-80, 88222,78606,25215,25236,A36797,A36801,26181,A36739,1245906,82073,1335906, 4115-0125,4115-0250,4115-0500,4115-1000,4115-2000,4115-2800,NC0012954,07-000-110,07-000-670, 0.5 mL MAX Efficiency DH10Bac Competent Cells*, 1 mL ExpiFectamine Sf Transfection Reagent, 500 mL Opti-MEM I Medium, with phenol red*, 24 Nalgene Single-Use PETG vented 125-mL Erlenmeyer Flasks**. pFastBac Dual Expression Vector from Thermo Fisher Scientific Description The pFastBac Dual vector features two promoters in a single vector for expression of two proteins simultaneously in insect cells when using the Bac-to-Bac Baculovirus Expression System (Cat. To streamline recombinant virus preparation, we Lemaitre et al. In performance tests against other suppliers’ media, the ExpiSf system demonstrated greater protein expression, delivering 3x more protein. Recently, BEVS has demonstrated particular utility in the commercial-scale manufacture of vaccines and gene therapy vectors. The cloning vector in our baculovirus expression system, pBV, is optimized for use with the baculovirus shuttle vector (known as bacmid) derived from the baculovirus strain AcMNPV (Autographa californica multicapsid nucleopolyhedrovirus), which has a 134 kb genome in its wildtype form. Many eukaryotic proteins undergo posttranslational … Insect cell culture media, baculovirus and host cell expression technologists around the world rely on Expression Systems for premium products, services and support. The ExpiSf Expression System is a complete chemically defined baculovirus-insect cell protein expression system that delivers superior yields and consistent performance run after run using a fast, streamlined workflow. 10359-016). Protein expression following baculovirus inoculation. Proven technology now with quick 5 minute TOPO Cloning procedure Our new Bac-to-Bac Baculovirus Expression Systems deliver quick, robust and proven method to produce recombinant baculovirus, expressing your gene of interest in insect Plasmid Maxiprep kit : ThermoFisher Scientific: K2100-06: For bacmid purification: Sf9 Cells: ThermoFisher Scientific: 11496-015: Insect cells: Grace’s Insect Cell Culture Medium: … The Baculovirus Expression System with Gateway Technology offers: Expression using the Bac-to-Bac technology (1) Destination vectors carrying the polyhedrin promoter for production of native (pDEST 8), N-terminal histidine fusion (pDEST 10), and N-terminal GST fusion (pDEST 20) proteins pDEST 10 vector containing a TEV protease cleavage site for removal of the fusion tag after protein purification (2) Be … The superior performance of the chemically defined ExpiSf system delivers consistent results across expression runs and over multiple cell passages. The BaculoDirect™ system accelerates research by eliminating these time-consuming steps, saving you precious hands-on time. A simple pauci-mannose type, the major N-glycan expressed in the original baculovirus-silkworm system, would be easy to modify. Baculovirus expression system: DH10B-T1R Competent cell: ThermoFisher Scientific: 12331-013: Competent cell for bacmid: TE buffer: In-house: Non-catalog item: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. Experience the first-ever chemically defined baculovirus expression system that can generate three times more protein compared to existing insect expression platforms. Moraes AM, Jorge SAC, Astray RM et al (2012) Drosophila melanogaster S2 cells … Consommables en plastique de culture cellulaire, Voir les liens pour Applications et techniques, Extraction et analyse de l’ADN et de l’ARN, Solutions pour les sociétés de biotechnologie, Recherche pharmaceutique et développement de médicaments, Industries pharmaceutiques et biopharmaceutiques, Spectroscopie, analyse élémentaire et isotopique, Développement du diagnostic préclinique au diagnostic compagnon, Logiciels de gestion et d’analyse de données de laboratoire, Consommables en plastique et matériel de laboratoire, Réactifs de culture cellulaire et de transfection, Colonnes de chromatographie, résines et filtres de centrifugation, Réactifs de laboratoire et produits chimiques, Fournitures, consommables en plastique et en verre pour laboratoire, Amorces/oligos, clonage et synthèse des gènes, Informatique de laboratoire à l’échelle de l’entreprise, OEM & Commercial SupplyLicences et offres commerciales, Certifications ISO du site de fabrication, Notions fondamentales en culture cellulaire Gibco, Lettres d’information électroniques et journaux, Plate-forme d’outils et d’utilitaires pour oligos, Données chiffrées utiles pour la culture cellulaire, Générateur de panels de cytométrie en flux, Outil Switch-to-Nunc pour les supports de culture, Calculateur de protocoles de transfection, BaculoDirect™ Baculovirus Expression System, First-ever, chemically defined expression insect system, ExpiSf Expression System smart start guide, ExpiSf: A chemically-defined expression system for enhanced protein production in Sf9 cells, Applications de bureau et applications mobiles, Culture volume (mL) for equivalent yield*. 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